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The K347R mutation did not increase transaminase activity.
The nprE aprA mutation did not lead to a significant increase in the CWB-LipB accumulation.
In addition, the H3K36R mutation did not affect total histone H3 levels (Supplementary Fig. 8).
But, this mutation did not result in change in amino acid sequence.
The nonclinical γ-secretase inactive PS1-D257A mutation did not alter gene expression in either cell line.
Addition of the K257R mutation did not however, increase the stability of the double mutant any further.
The new mutation did not simply reverse the mutation that had originally turned the microbe's ancestors into cheaters.
We find mnb mutation did not reduce the phosphorylation of dynamin, Endo, or Dap160 (Supplementary Fig. 3h).
The mutant formed normal-architecture biofilms but showed decreased penicillin G resistance, although the mutation did not affect planktonic cell resistance.
The P726S mutation did not reduce the susceptibility of 5- and 14-day-old mice to 633 and TE viruses in vivo.
Heterozygous rb1 +/− adults carrying a germline mutation did not develop tumors.
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