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Serial dilution and plating experiments showed that the erg26 mutation did indeed result in an elevated petite frequency at both 25° and 30° (Table 2).
We found that the gltA1 mutation did indeed improve competitive fitness when it was added to a clone isolated from the LTEE population around the time when it appeared, particularly in growth medium supplemented with acetate.
Cells containing wild-type RelA showed a robust induction in Cxcl2 mRNA levels, reaching levels similar to that found basally in the T435D cell line, suggesting that this mutation did indeed mimic the effects of TNFα stimulation for this NF-κB gene target.
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Thus the OPC culture experiments show that the R100 mutation does indeed impair p75NTR signaling in the context of mature NGF, but does not do so when present in the context of hproNGF.
This confirms that the knock-in mutation does indeed ablate SPAK activity.
3) The paper would be strengthened by evidence that the F876L mutation does indeed occur in PCa patients undergoing ENZ therapy, and, although it may be too soon for ENZ-resistant patients to have emerged, it would be worth discussing this as a future direction, because these patients would obviously be candidates for treatment with the second-generation drug reported in this paper.
We have designed a double mutant (N78V/H81L) located in the coiled-coil interface of the leucine zipper domain and we demonstrate that these mutations do indeed increase the stability of the protein.
In certain populations, one or a few mutations do indeed capture the majority of CHEK2 variants associated with cancer risk.
For humans it has been suggested that such mutations do indeed occur and have specifically been fixed in individuals of populations living at higher latitudes indicative of providing an adaptation to the colder climates [ 33].
If the mutations at R37 did indeed take place within the last eight generations in the 21 examined lines, then the mutation rate at R37 could be as high as 3×10−2 per generation.
She had to wait 18 months before she was able to obtain the test through a grant, at which point she learned she did indeed carry a mutation.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com