Sentence examples for mutation detection system from inspiring English sources

Here we developed a DNA mutation detection system based on CRISPR/Cas9 that can detect gene mutation efficiently even in a low-frequency condition.

Identically, using the lacI mutation detection system, the mutant frequencies were 6.4±3.1×10−5 and 5.8±2.0×10−5.

By using the mtDNA point mutation detection system of Series A (29 mutations) and Series B (32 mutations), we screened a total of 3103 mutant sites in 107 DNA samples for Series A and 13,101 mutant sites in 397 DNA samples for Series B. We succeeded in determining 99.4% (Series A) and 99.6% (Series B) of the targeted mutant sites by use of the system.

The electrophoresis was run in 1× TAE buffer for 10 h, (100 V, 60 °C) in Universal Mutation Detection System apparatus (Bio-Rad, Dcode, USA).

Denaturant gradient gel electrophoresis of amplified PCR products which were generated from DNA extracted from wound tissue debridement samples were performed on acrylamide gels in a DCode™ universal mutation detection system (Bio-Rad) according to the conditions described by Rasiah et al. (2005).

The DGGE analysis was performed in a D-code Universal Mutation Detection System (Bio-Rad, Hercules, USA) in a 7% polyacrylamide gel containing a linear 35 55% denaturant gradient with a constant voltage of 150 V at 60 °C for 5 h.

In conclusion, this method allows the analysis of separated alleles of heterozygous patients, avoiding possible allele-interfering events affecting other mutation detection systems [ 29].

High throughput technologies and more sensitive mutation detection systems in the DNA diagnostic laboratories have led to an increasing number of sequence variants in the major cancer-predisposing genes for which the clinical significance is unknown.

Though collecting surgically resected metastatic tumour tissues is often difficult, circulating tumour cells may be a useful alternative DNA source for highly reliable and sensitive mutation detection systems such as the ARMS/Scorpion method for further analyses.

Such point mutations are easily detected by mutation-detection systems such as the CEL1 nuclease assay or high-resolution melting curve (HRM) analysis [ 3, 4].

Here, we combined the JAK2 V617F and MPL W515L/K mutation detection with a multiplex system, which reduced the total processing time of molecular diagnosis of MPNs.