Sentence examples for mutation detection gel from inspiring English sources

For mutation detection gel analysis (MDGA) optimal product size was 300 bp so PCR amplification of exon 2 was split into 2 regions.

The mix was denatured at 95°C for 10 minutes and kept on ice until loading onto 0.8XMDE (Mutation Detection Enhancement) gel (Flowgen).

The mix was denatured at 95°C for 10 min and kept on ice until loading onto 0.8×MDE (Mutation Detection Enhancement) gel (Flowgen), both with and/or without 10% Glycerol.

For point mutation, previous research using 'hydrolink mutation detection enhancement gels' cannot detect all types of the mutation via heteroduplex analysis.

These ISR markers can be assayed on mutation detection enhancement (MDE) gel for detection of polymorphism.

The remaining exons and their splice sites were screened by Heteroduplex analysis (HA) using Mutation Detection Enhancement (MDE) gel solution (Cambrex), essentially as described by Claes et al. [ 13].

Fragments were run both on a non-denaturing 6% polyacrylamide gel at 6 W (3000 V, 300 mA) for 16-18 hours and on a MDE™ gel (Mutation Detection Enhancement, BMA products, Rockland, ME) for 8 hours at room temperature.

Amplified PCR products were tested for indels at the target site with the SURVEYOR mutation detection kit for standard gel electrophoresis (Transgenomic, Omaha, NE), as per the manufacturer's instructions.

We performed the Surveyor nuclease assay using 20 µL of unpurified PCR products incubated with 1 µL of Surveyor nuclease (Transgenomic SURVEYOR mutation detection kit for standard gel electrophoresis), 1 µL of Surveyor Enhancer, and 4 µL of 0.15 M MgCl2 in a 50-µL reaction.

For polymorphism screening and mapping, amplification products were separated on mutation detection enhancement (MDE) acrylamide gels to display single-strand conformation polymorphisms (Martins-Lopes et al. 2001).

The proportions of ATM heterozygotes were determined using the denaturing gradient gel electrophoresis mutation detection method.