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In a mutation detection experiment, a sample of unknown mutation status is run in individual real-time PCRs with one assay that targets mutant alleles within a gene and the corresponding gene reference assay.
Oligonucleotides for the barcoded Illumina adapters used in the mutation detection experiment were as follows (barcode in lower case): Control, adA: P-atcacAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG adB: ACACTCTTTCCCTACACGACGCTCTTCCGATCTgtgatCATG The oligonucleotides were ordered as in desalted quality and were not modified except for phosphorylation of adA.
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However, the maximum fragment length and sequence divergence between homeoloci where HRM remains useful for SNP or mutation detection is unknown and further experiments are required.
JC, BT, SH, and LC designed experiments and interpreted the mutation detection data.
Apparently, Fermilab scientists were performing a collision detection experiment when other unusual collisions were detected.
CDH1/E-cadherin mutation detection rates vary between 12% and 83%.
A rapid and reliable mutation detection assay has been developed.
Fig. 8 Corridor detection experiment results.
Fig. 6 Corner detection experiment results.
HY and WC performed the plasmid construction, plant transformation and mutation detection.
The MLPA method was shown to be reliable for mutation detection and identified five published and 12 new mutations.
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