Sentence examples for mutation detection due from inspiring English sources

Homogeneous methods, exampled by real-time PCR, have proved to be very useful in mutation detection due to easy automation, high throughput, and low risk of post-PCR contamination [3], [4], [5].

Use of a GFP plasmid-based mutation detection system may enhance the sensitivity of mutation detection due to i) multiple copies of the frameshift-containing GFP gene; pMF230-based plasmids have a copy number of approximately 50 100 per P. aeruginosa cell (M.

This could be due in part to the insensitive techniques that clinicians use for KRAS mutation detection or due to the presence of other undetected mutations in different genes in the RAF MEK ERK kinase pathway.

Mutation detection is complex, due to the large gene size, the large variety of mutations and the presence of pseudogenes.

It was chosen for mutation detection in this study due to the high probability that missense mutations in this exon will have deleterious effects on the enzyme activity, and it has a large number of TGG and CAG codons that can mutate to premature stop codons.

These observations suggest that the lack of nonsense mutation detection in these amplicons is due to limitations of the detection method, which may be related to features in the DNA sequence.

Over the last few years, next-generation sequencing (NGS) has become a popular strategy for genotyping, enabling more precise mutation detection compared to traditional methods due to its high resolution and high throughput [ 3].

Thirdly, due to the fact that mutation validation is time consuming, especially for multi-copy genes, and that the uneven pooling and PCR efficiency will all cause some mutations to be easier to detect than others, our mutation detection strategy focused on efficiency instead of detecting all possible mutations.

This is probably due to insensitivity of the mutation detection method or because other mechanisms of inactivation (e.g. methylation) were active.

MDA works best for mutation detection but is not sufficient for copy number analysis due to moderate amplification bias and non-uniform genome coverage.

Therefore, the detection rate was increased by 21%, mainly due to dHPLC scanning, achieving a mutation detection rate of 64.5% (40/62).