To identify proteins functionally connected to the lagging strand DnaE polymerase, a search was conducted for extragenic suppressors of four dnaE thermosensitive (Ts) mutations (named dnaE2.2, dnaE2.4, dnaE2.6 and dnaE2.10, see Table 1 for mutation details).
Sequencing of all 23 exons of the PTCH1 gene indeed identified frameshift mutations in 4 tumors of cluster B, but not in any of the remaining tumors (P = 0.009) (Figure 1A and Table S1 for mutation details).
In the six MM cell lines, selected mutations in RTKs and adhesion molecules were correlated with mRNA expression, chromosomal copy number changes and copy neutral loss of heterozygosity (Tables 3a and b, for mutation details see Supplementary Table S14).
Our results overall strengthen the overwhelming necessity of cost-effective and practical methods for EGFR mutation detailed characterization [ 7] for NSCLC patient management, even when dealing with small amount of tumoral tissue.
Clicking above any of these images loads an interactive page with visually intuitive presentations of structure, scoring, mutation, and sequence details for that trial (Figure 15B).
Details for diagnoses, number of mutations analysed, methods used, and other specifics for individuals with found mutations within the three groups are shown.
The details for each of the samples and each mutation are presented in two separate summary pages (not shown).
For each mutation all details on the sample like patient age, gender, histology and tissue are available.
Since a molecular structure of TGR5 was not available, a structural model was generated by comparative modeling in order to predict the role of the nonsynonymous mutations (for details see Results S1).
