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It is compatible with the robustness of protein structures to mutation described by the notion of minimal frustration [48].
To facilitate the investigation of intragastric infections of mice, the inlAm (inlAS192N,Y369S) mutation described by Wollert et al. [61] was introduced into a wild type 10403S strain NF-L11244) and the prfA G145S mutant by electroporation, allelic exchange, and plasmid curing of the plasmid vector pHEL-913 (pKSV7-inlAm, a kind gift of Dr. Helene Marquis, Cornell University, Ithaca, NY) [38].
For each RET variant, matrices of delta values for each biochemical property of the substituted amino acid were calculated by Python scripting and the resulting mutation described by an array of variables archived using SQL - where each matrix corresponds to the absolute value of the difference between the value of the property in the amino acid present in the wild type and the one in the mutant.
Murine embryos that harbour the Brca1 mutation described by Gowen et al [ 14] typically survive to embryonic day 9 or 10.
The R485L mutation found in this study differed from the R485C mutation described by Feuerriegel et al. [ 17], which had polymorphisms within codon 485.
To select genes that are the most likely candidate cancer genes, we used the mechanisms of retroviral mutation described by Jonkers and Berns, 1996 (Table S2).
Similar(51)
Indeed, none of the somatic mutations described by Jones et al were found in our analyses and viceversa.
Of the original four families with GATA2 mutations, described by Hahn et al.
This change resembled the effect of catalytically inactive Twinkle mutations described by Wanrooij et al. (22).
These 2 vectors incorporated mutations described by Hoffman MA and Palmenberg AC [ 40] into the attenuated IRES [ 44, 45].
However, the G120R, G120P, A121H and A121P mutations described by Olesky et al were not found, suggesting differences in the amino acids replacement by natural and artificial mutations.
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