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mtDNA mutation density was significantly higher in smokers than in non-smokers [3].
A soybean mutant library with a high mutation density was developed.
The overall mutation density was calculated to be ~1/526 kb for the EMS generated mutant population (Table 3).
Specifically, the mutation density was positively correlated to the density of HU-activated genes and FIS-repressed genes (Table 1, Figure 4B).
The mutation density was estimated to be approximately 1 mutation per 74 kb (range, 43 106 kb), which was much higher than expected.
Mutation density was estimated as the total number of mutations divided by the total number of base pairs screened (amplicon size × individuals screened).
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MuLV has integration biases that vary substantially throughout the genome such that mutation density is insufficient to differentiate selected driver mutations from passengers.
This mutation density is well suited to high-throughput mutation discovery.
The presence of multiple loci with high mutation density is therefore a deaminase specific process.
This mutation density is higher than those found by earlier studies based on TILLING [ 7, 35– 35].
Only one such region of high mutation density is seen in the EMS treated clones, that of the CAN1 gene.
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