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The substantial mutation density suggests that soybean should be an amenable subject for a high-throughput TILLING service.
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Mate-finding probability was unaffected by population density, suggesting the absence of mate-finding Allee effect, and population growth rate increased with decreasing density, suggesting negative density-dependence.
The relatively high UV dose resulting in the survival of only 0.4 to 2% of the cells and the fact that during segregation analyses at least two out of five gox mutants contained two mutations affecting the phenotype suggested a high mutation density in the screened population.
We estimated the mutation density based on Greene et al. [20] to 1 mutation every 573 kb.
False positives make phenotype sequencing much harder by spuriously increasing the apparent mutation density per genome.
mtDNA mutation density was significantly higher in smokers than in non-smokers [3].
The remaining population had a mutation density of ~1/550 kb.
A total of five mutations were identified resulting in a calculated mutation density of 1/526 kb.
This mutation density is well suited to high-throughput mutation discovery.
We estimated an overall mutation density of 1 mutation per 40 kb screened.
A high mutation density (1 mutation per 74 kb) was achieved by repeating the EMS treatment.
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