Sentence examples for mutation created by from inspiring English sources

We identified an embryonic stem (ES) cell line with an EpCAM (tacstd1) mutation created by gene trapping, and used it to generate EpCAM-knockout mice which we then characterized.

The PI3K haploinsufficient SHIP−/− mice analysed in this study possess a mutation in the p85 regulatory subunit of PI3K 48 and a SHIP mutation created by Helgason et al. 41 All mice were between 6 and 13 weeks of age at time of sacrifice.

Mutations created by QuickChange were confirmed by sequencing (Sanger method) the entire MLH3 open reading frame.

In addition, the mutations created by amplification errors could add more diversity to the library.

Digested products were resolved by electrophoresis in 1.5% agarose gels; mutations created by ZFNs were evidenced as undigested PCR products.

One of the mutations created by the cry8Ka1 gene shuffling procedure is localised in loop 3 of domain II in Cry8Ka5.

The mutations created by the eight sgRNAs were predominantly very short indels (56.5% single or double base pair indels) with a preference for single base pair insertions.

When the mutations are introduced by error-prone PCR, the Poisson distribution is an excellent approximation to the true theoretical distribution of mutations created by error-prone PCR [ 40, 41] provided that μ is much less than the number of PCR doublings, as is the case in all of the experiments in the current work.

The E675A catalytic mutation was created by mutating codon 675 gAa to gCa in pch cloned into pUC18Tmini-Tn7T.

D474N mutation was created by PCR as described [25].

The RasV12 point mutation was created by PCR-based mutagenesis of the pMK33FlagHis-Ras1 vector.