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The V306A mutation causes no apparent alteration to Dyrk1A activity except for the reduction in ATP affinity.
The mutation causes no change in amino acid.
Thus, the eif3h mutation causes no dramatic defect during the first stage of post-meiotic pollen development, the stage that encompasses the two subsequent mitotic divisions that result first in one vegetative nucleus and then the two sperm nuclei [34].
If the identified mutation causes no amino acid substitution, it is necessary to return to the primer design step to amplify an alternative region.
Co-sedimentation experiments also supported this notion that the K1647D/K1650D mutation interferes with co-pelleting of the MyTH4 FERM domain with microtubules but the F2002K mutation causes no interference.
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Therefore, expression of the hnRNPA2/B1 gene was only reduced to about half of wild type levels, and the mutation caused no obvious phenotype in mice.
A SET1 deletion was previously shown to impair rDNA silencing, but this mutation caused no detectable defect in Net1 and Sir2 recruitment to rDNA [ 22].
These results are consistent with the finding that AZT resistance mutations cause no difference in ATP binding [15], [16].
The charge engineered variant's two mutated amino acids exhibit stabilizing interactions with adjacent native residues, and from a global perspective, the mutations cause no gross structural perturbations or loss of stability.
Q2′N, T6′A and T6′S mutations caused no change to the GABA EC50 values.
While some single mutations cause no measurable changes in fitness, others can change the function completely or make the protein nonfunctional.
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CEO of Professional Science Editing for Scientists @ prosciediting.com