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To assess macular photoreceptor abnormalities in eyes with fundus albipunctatus with RDH5 mutation by using adaptive optics scanning laser ophthalmoscopy (AO-SLO).
Progression-free survival rate for tumors positive for the germline mutation was compared with those negative for the mutation by using the Kaplan-Meier method.
Enrichment for the mutation by using MboII restriction digestion allowed the detection of mutant present at 1% or less.
18 discovered a resistant ALK mutation by using pleural effusion cell DNA obtained from an EML4-ALK lung cancer patient who acquired crizotinib resistance.
Patients from the F09 and F13 families had also been screened for detection of the RHO mutation by using direct sequencing in a previous study [ 22].
Detection of single based genetic mutation by using oligonucleotide probes is one of the common methods of detecting single nucleotide polymorphisms at known loci.
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Finally, colony PCR reaction was performed to verify the success of the mutations by using oligonucleotide primers 425-TT-BamHI-FW/426-TT-EcoRV-RV as referred in Table 2.
We have developed a web server for predicting the protein stability changes upon single mutations by using fuzzy query mechanism and it is available at http://bioinformatics.myweb.hinet.net/fqstab.htm.net/fqstab.htm
We study the outcome of immune escape mutations, by using two-strain dynamical models applied to the resident and the mutant antigenic clusters defined here above.
The isolates were also examined for gyrA and parC mutations by using primers and methods described (7 ).
2- Denaturing high-pressure liquid chromatography (dHPLC) was used to screen NRAS mutations by using a Transgenomic Wave machine (Transgenomic, NE, UK).
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