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A second allele was generated by targeted mutation by introducing a stop codon just upstream of the transmembrane domain.
Because epithelial tumors of some tissues are frequently associated with Kras mutation and those of other tissues are rarely associated, we tested the hypothesis that some tissues are refractory to the effects of Kras mutation, as opposed to being susceptible to its effects but rarely undergoing its mutation, by introducing the mutation into multiple tissues.
We have also constructed a quadruple mutation by introducing an additional P223G mutation.
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The enzyme produced by SD1 is known to control a biochemical pathway that promotes growth in the stems and leaves of the rice plant, so the team measured the effects of different SD1 mutations by introducing genes with those mutations into bacteria and seeing how much enzyme was produced.
In step 4, parts of solutions are changed by the mutation operation by introducing new genetic material into the population.
The variants with multiple mutations were obtained by introducing additional mutation(s) in a given variant, using the overlap PCR workflow described above.
Recombination may contribute to rate variation among loci by introducing mutations [ 27, 28].
The double mutant was obtained by introducing the mutation MUT2 in the pCdx2-1luc MUT1.
A mutated sublibrary was created by introducing random mutations in the KM10 scFv gene.
Individual roles of these AAA+ modules in ClpB have been studied by introducing mutations to the Walker motifs.
A similar effect was observed by introducing mutations at the same positions in the genotype 2a JFH-1 strain34.
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