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Éric Déziel (personal communication) confirmed the mvfR phenotype of PA7, and complemented the mutation by adding a plasmid containing the wild-type mvfR gene from PAO1.
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We have clarified our rational for looking at activity enhancing mutations by adding these sentences to the results section: "Our ability to link protein folding to antibiotic resistance gives us a unique opportunity to select for activity enhancing mutations in a chaperone.
In summary, we demonstrated the ability of 2 pairs of TALEN to induce GSV including deletion, duplication and inversion of a chromosomal fragment as large as 8.9 Mb, which is about one third of the entire chromosome, and developed an efficient strategy to reduce the unwanted mutations during structure variation by adding ssODN.
Another previous study also attempted to enhance mutation efficiency by adding limited amounts of circular plasmid in PCR reaction [ 21].
The mutation scores are obtained by adding sample mutation scores over all samples followed by normalization to 1.
We enhanced the analytical specificity of the mutation-specific assay by adding a proprietary co-solvent, Q-Solution, to the PCR reaction.
The mutagenesis conditions were tuned to induce ∼2 mutations per amplicon by adding 1130 ng of plasmid template (Salverda et al. 2011).
Simulated data are obtained by adding mutations under a stepwise mutation model [ 56] for short tandem repeats (STRs) and an infinite sites model [ 57] for sequence data.
We analyzed the retinas of the knock-in Ter349Glu RHO mice, which carry a human rhodopsin gene with a read-through mutation that was generated by adding 51 amino acids to the 1D4 epitope.
As one moves away from this genotype by adding mutations, imagine that the ratio of deleterious to neutral mutations stays constant, as does the average effect of a deleterious mutation.
They do so by adding mutations to the viruses as they replicate.
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