Sentence examples for mutation back to from inspiring English sources
Re-introduction of the F857-L mutation back to the genome of Vero-adapted IBV (p65) showed rescue of virus containing the F857-L mutation.
This could occur via the intra-genetic reversion of the WS wspFS301R mutation back to the SM (wild-type) sequence, or via inter-genetic reversion at other loci involved in the regulation or expression of cellulose.
This occurs if PCR reverts a real mutation back to the allele length of the progenitor.
Of note, we did not detect a reversion of the R292K mutation back to the wild-type in the lungs of mice inoculated with SH5190 R292K during the observation period; in parallel, no R292K mutation was found in mice inoculated with the wild-type virus, as evidenced by the SNP qPCR assay (data not shown).
While all of them significantly improved signal-to-noise ratio, they do not apply well to TILLING because of their high cost, complex of PCR and ligation strategies, and/or their inability to track a mutation back to a specific mutation carrier.
In the case of the water buffalo, which also belongs to Bovini, the existence of the full PALI1 is probably due to a later reversal mutation back to the original sequence as it lacks the Bov-A2 element, like its tribal fellows the cows.
As a result, this 'immune relaxation' might allow the reversion of costly viral escape mutations back to a fitter wild type state.
This is demonstrated clearly by reversion of mutations back to the fitter wild type (WT) sequences upon transmission to new hosts not able to mount the same immune response [3], [4], [5].
A mutagenesis strategy was designed to partially revert these mutations back to the original sequence, obtaining 2 novel antibodies, scFv43 M1 and scFv43 M2.
The surviving subpopulations either diverged into separate species and lineages or channeled their TE-produced mutations back to the meta-population by crossbreeding.
Further supporting the importance of the recA, dnaB, and yfjK mutations, conversion of each of these mutations back to the wild-type allele causes a substantial loss of IR resistance.
