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The paucity of reports of the Δexon14 variant in MPN patients most likely derives from the fact that JAK2 mutation assays typically rely on DNA rather than RNA.
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To do so, we performed site mutation assays (Figure 4).
As our JAK2 mutation-specific assay typically has an amplification efficiency approximately equal to 1.7, this will be close to 1 Cq earlier.
They also speculate that the types of mutations induced by arsenic are large chromosomal losses that are hard to detect with conventional mutation assays.
In addition, the mutation assays are less labor intensive.
In principle sequencing and the mutation assays are similar techniques.
Each assay consisted of an allele-specific SuperSelective primer45 and a common primer shared by the normal and mutation assay.
The mouse lymphoma assay (MLA) is the most widely used in vitro mammalian gene mutation assay.
M.D.S. worked together with R.S.H. on all aspects of these studies and did most of the experiments; M.B.B. and M.L. established the TK mutation assay and the in vitro DNA deamination assays, respectively; J.L. performed the virus infectivity experiments.
Cha, R. S., Zarbl, H., Keohavong, P. & Thilly, W. G. Mismatch amplification mutation assay (MAMA): application to the c-H-ras gene.
Ames reverse mutation assay.
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