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The FGFR3 mutation assay needs little DNA, has a high performance rate on DNA isolated from formalin-fixed paraffin embedded tissue (FFPE DNA) and urine and was found to be highly reproducible.
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Each assay consisted of an allele-specific SuperSelective primer45 and a common primer shared by the normal and mutation assay.
The mouse lymphoma assay (MLA) is the most widely used in vitro mammalian gene mutation assay.
M.D.S. worked together with R.S.H. on all aspects of these studies and did most of the experiments; M.B.B. and M.L. established the TK mutation assay and the in vitro DNA deamination assays, respectively; J.L. performed the virus infectivity experiments.
Cha, R. S., Zarbl, H., Keohavong, P. & Thilly, W. G. Mismatch amplification mutation assay (MAMA): application to the c-H-ras gene.
Ames reverse mutation assay.
The reverse mutation assay (Ames test [24]) is a common procedure involving the reverse mutation of histidine dependent Salmonella typhimurium and Escherichia coli strains.
Mutagenicity of the cordycepin was tested by bacterial reverse mutation assay (Ames test) using Salmonella typhimurium strains TA98 (to detect frame shift mutation).
This was 4% for the KRAS mutation assay.
Additional studies are needed to determine if mitochondrial mutation assays provide new or complementary information for estimating cigarette smoke exposure at the cellular level or as a cancer risk biomarker.
While the clinical relevance of newly identified mutations needs to be further validated, the speed with which cancer genomes can now be sequenced underlines the need for continuous updating of the mutation assays used in the clinics.
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