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Real-time allele-specific oligonucleotide PCR was performed on available WM DNA using qBiomarker Somatic Mutation Assay for MYD88_85940 (SABiosciences, Qiagene, Hilden, Germany) according to the manufacturer's protocol.
We selected the T-cell cloning assay as the HPRT mutation assay for our study; the method has been described previously (Ma et al. 2000).
Results were convincing; the SPACE-HRM detection system might be a good choice for one-step mutation assay for its highest sensitivity.
We carried out an in vitro mutation assay for [GT/CA]n repeats, using two-unit repeat loci from their flanking sequences as monitors of background slippage.
Many toxicological methods have been invented to test for the presence of gene mutations and chromosome aberrations due to environmental pollutants, such as the Salmonella reverse mutation assay for mutagenic activities and the comet assay for chromosome damage [ 1].
This study contributes to the validation of the Pig-a endpoint by comparing its responsiveness to that of the well established lacZ TGR mutation assay for the well-known mutagenic carcinogen benzo[ a]pyrene (BaP).
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One patient sample is tested by one control assay and seven mutation assays for a total throughput of 10 samples per 96-well plate.
One widely used and well-validated system for assessment of gene mutations in vivo is the transgenic rodent (TGR) mutation assay system (for review see [Lambert et al., 2005; OECD, 2009]).
Spi− selection based on deletions extending into or through both the redBA and gam genes is an efficient mutation assay system for detecting small to kilobase-sized deletions in different cells, organs, and tissues (Nohmi and Masumura 2004).
The reverse bacterial mutation assay was performed for screening the mutagenic potential of the plant extracts as described elsewhere [ 4].
The mutagenicity of the particulate fraction of mainstream smoke has mostly been tested in the plate incorporation version of the Salmonella typhimurium reverse mutation assay, e.g. for the benchmarking comparison of commercial cigarettes (Roemer et al., 2004; Rickert et al., 2007b) or for the assessment of changes in cigarette filter design (Gaworski et al., 2009).
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