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Each of the cell lines harbored a TP53 mutation as verified by sequence analysis (Table 2).
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This substitution was due to an unintentional mutation introduced at the cloned sequence, as verified by DNA sequencing.
The location of the mutation as well as the zygosity could then be verified.
Extra stability beyond the threshold confers no direct benefit on a protein's function, but it does increase the protein's mutational robustness by allowing it to tolerate a wider range of destabilizing mutations (as has been experimentally verified for three different enzymes [ 3- 5]).
Finally, accession numbers, mutation and mutation positions are verified, and attempts are made to manually check and include missing information such as chromosomal location, accession number/s and valid HGNC gene symbols.
The mutation was verified through molecular analysis.
Each mutation was verified by DNA sequencing.
The correct mutation was verified by sequencing.
The presence of the desired mutation was verified by sequencing.
The presence of the mutation was verified by direct sequencing.
Introduction of the desired mutation was verified by plasmid sequencing.
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