Sentence examples for mutation as previously from inspiring English sources

All mice were genotyped for the GarsC201R mutation and Dync1h1Loa mutation as previously described [22], [25], [26] at approximately 28 days of age.

were transfected (Amaxa nucleofection, Amaxa Inc., Gaithersburg, MD) with plasmids containing NS5A sequences or with control sequences including the vector control expressing GFP, or with a vector that contains NS5A sequences with a single base insertion to create a frameshift mutation as previously described [33].

Clinical isolates from the same patient were identical by MLVA on two occasions (C5 & C6 and C11 & C13), but were divergent by one to three loci in an ancestral topography during all other cases (C2 & C3, C7 & C8 and C9 & C10), consistent with in vivo mutation as previously reported [16].

This can be explained by the fact that Q297V mutation, as previously proposed (37, 40), should make OPA1 constitutively active by mimicking the GTP-bound state.

Renal cell carcinoma xenografting was carried out with mice (Animal Resource Centre) that are homozygous for the SCID mutation as previously described (Huynh et al, 2006).

Genotyping of the Hmx1 dm and wild-type Hmx1 (Hmx1 +) alleles was performed by real-time PCR using oligonucleotide primers having 3′ termini at the point mutation that characterizes the dmbo mutation, as previously described (Quina et al., 2012).

The BRAF gene mutation – which as previously described is integral to melanoma pathogenesis – has been investigated as a prognostic marker too.

After heat-induced antigen retrieval, sections were incubated with the primary monoclonal IDH1- R132H antibody (clone H09, 1 10 dilution; Dianova, Hamburg, Germany) that specifically recognizes IDH1-R132H mutation status, as previously described [ 11].

In our study, we showed that nuclear β-catenin expression was independent of APC tumour suppressor gene mutation, as was reported previously (Kobayashi et al, 2000).

Another possibility is that the high levels of expression we observed for key molecular chaperones may help to suppress the phenotypic effect of these deleterious mutations, as has previously been reported for intracellular bacteria [ 46].

This method correctly identified all 10 samples that were 3243A>G mutation-positive, all 4 samples that were 8344A>G mutation-positive, and all 30 samples that were negative for both mutations, as previously identified by traditional gel-based methods.