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An increase in the mutation rate, on the other hand, is not expected to produce any significant variation in the d N/d S ratio since increasing the rate at which mutation are introduced in populations will rise up both synonymous and nonsynonymous changes to a similar extent (Itoh et al. 2002).
Similar(59)
The T315I mutation was introduced by site-directed mutagenesis.
Subsequently, a R419L mutation was introduced into purA to reduce the flux from IMP to AMP.
In brief, the S89G mutation was introduced in exon 6 using an overlap extension-PCR method.
The R217Q mutation was introduced by site-directed mutagenesis.
The AXXLL mutation was introduced by a fusion PCR approach.
The point mutation was introduced with the QuickChange XL Site-Directed Mutagenesis kit (Stratagene).
DNA sequencing was performed to confirm that no undesired mutation was introduced by PCR.
The D174H mutation was introduced by PCR site-directed mutagenesis as described previously [55].
The R217Q mutation was introduced in VSFP2A by site-directed mutagenesis.
To create pFOX4, the P621G point mutation was introduced to the pFOX1 sequence.
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