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Nevertheless, the cell lines described in the databases as carrying a single heterozygous mutation appeared very unstable in this state or were potentially misclassified.
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Surprisingly, we found that mutations appear very rapidly after transfection: we were able to observe EGFP positive cells within 3 hours of transfection (Figure 4).
Trinidad appeared very loose.
She was smiling and appeared very happy.
He appeared very sincere".
She appeared very distressed when describing this.
All the karyotypes appeared very rearranged.
However, when a selectively advantageous mutation appears, it very quickly sweeps through the population.
The generation of novel genes by mutation appears a very unlikely event and the chance of generating a functional gene by random mutations decreases with the length of a gene.
Moreover, 80% of sporadic colorectal cancers feature APC gene somatic mutations, which may appear very early during colorectal carcinogenesis [ 17].
On current evidence in some 83 BRCA2 mutation carriers prognosis does appear very similar to sporadic breast cancer and may well reflect the very similar tumour characteristics [ 17].
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