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This vector was used to re-introduce pglB activity to cells to test pACYC pglΔ pglB mutation and serve as a control of pjexpress401 pglB∗.
We and others have characterized longevity of core metabolic genes in the face of a mutational "ratchet" (that over hundreds of millions of years would be capable of decimating eukaryotic exons), and suggested existence of adaptive mechanisms that regulate DNA mutation and serve to promote long-term genomic survival (Gabriel et al. 1993; Lynch 2010; Koonin 2012; Hill et al. 2014).
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The female F1 mice are carriers of the B6.gp91phox mutation and served as the controls for the male mice.
The rtL180M mutation usually occurs concurrently with the rtM204V mutation and serves as a compensatory mutation [ 11].
An animal model of E1α deficiency could provide insight into the pathological consequences of mutations and serve to test potential therapies.
These data provide essential insights into the underlying molecular mechanisms upon mutations and serve as caveats for future functional gene knock-out studies.
In terms of functional analysis, our computational modeling and gliding assays were performed on 3 of the de novo mutations and serve to represent the overall de novo sample.
These results explain the cold-sensitive phenotype of the I707L mutation in KlenTaq1 and serve as an example of a large conformational change affected by a conservative mutation.
Thus, the mutations might reduce antigenicity and serve as an alternate escape mechanism to loss of antigen or MHC expression.
In addition, there are many databases that store and serve somatic mutation data for single genes (see http://www.hgvs.org for an extensive list).
mind bomb mutants have compromised Notch activity due to mutations in the mib gene and serve as a unique resource to study the role of Notch signaling on various developmental processes [27], [29].
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