Sentence examples for mutation and screening from inspiring English sources
Their goal is to optimize the distribution of metabolic fluxes towards the product of interest, either directly (e.g. FBA, MOMA or ROOM) or indirectly by coupling it to another characteristic (e.g. OptKnock) that facilitates further strain improvements via mutation and screening.
A well-controlled lethal rate is fundamental for effective mutation and screening of the mutants.
To investigate the accumulative effect on TGase production by iterative ARTP mutagenesis, the mutation and screening results for each round were collected and compared.
In order to examine if random mutation and screening could improve this, a DNA-shuffling method would be used to generate a mutant LDH and recombinant LDH genes which will be later used for transforming Escherichia coli.
PC-3-7 was derived from QM9414 via five stages of mutation and screening and there are at least 154 SNPs within the promoter, coding region, and terminator regions of the genome when compared to wild-type QM6a.
Confirmation and segregation of the c.42+1G → C mutation and screening of 20 additional COX-deficient probands for mutations in NDUFA4 was assessed using standard PCR-based sequencing.
We randomly picked five transformants per mutation and screened them for cell growth and production of active HRP in shake flasks.
The chapter explores the theoretical basis for each process in directed evolution: mutation, recombination, and screening.
The theoretical grounds underlining the design of such libraries are described, including the mutational load, the ratio of beneficial versus deleterious mutations, and screening capacity.
The use of synthetic oligonucleotides for recombination circumvents any cloning steps and provides maximum flexibility for introducing mutations and screening markers.
Accumulated evidence indicates that FLT3-ITD functions in approximately 20% of AML as driver mutations, and screening FLT3-ITD could benefit to select the more accurate prognostic criteria and therapeutic strategies.
