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Structural observations are further validated by mutation and isothermal titration calorimetry (ITC) assays.
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We used PCR stitching and isothermal assembly to generate mutated versions of these constructs.
GOOD: Laboratory techniques include RT-PCR, commercial-scale lyophilization, electrophoresis, and isothermal titration calorimetry.
The resulting mutational constructs of ALR2 (eight point mutations and one double mutant) were probed for their influence towards ligand selectivity by X-ray structure analysis of the corresponding complexes and isothermal titration calorimetry (ITC).
Both adiabatic and isothermal compressibility is discussed.
The chromatographic conditions were as follows: 40°C isothermal for 5 min, 15°C/min up to 300°C and isothermal for 25 min.
Interactions between MA and DNA were studied by 1HN/15N chemical shift perturbation mapping, PRE measurements and isothermal titration calorimetry (ITC).
Heating and isothermal amplification were separately performed on simple equipments including water bath and heating block.
Two main approaches for WGA have been developed: thermocycling protocols and isothermal amplification methods.
There are two categories of commonly used WGA methodology: PCR amplification and isothermal DNA amplification.
NMR binding, fluorescence titration assays and isothermal titration calorimetry thermodynamic analysis.
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