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All patients were analysed for presence of EGFR and KRAS mutation and for ALK translocation: this analysis included 56 (44.8%) EGFR mutated, 29 (23.2%) KRAS mutated, 10 (8.0%) ALK translocated and 30 (24.0%) EGFR/KRAS/ALK wild type, defined as triple negative.
Next, they tested 50 healthy adults for both the mutation and for above-average IgE levels.
The selection criteria for MKD (N = 12) was the detection of only a single mutation and for CAPS (N = 76), it was evocative signs, i.e. recurrent fever, urticaria, arthralgia and sometimes neurosensorial deficiency.
The analytical selectivity of MAP was >1010 for an insertion, >109 for a tandem base mutation and for two indels, and >108 for a doublet (two single base substitutions separated by 5 bases) (Fig. 4, Table S3).
Although the empirical substitution matrices represent the average tendencies of substitutions over proteins and species and may lack gene-level resolution [15], [16], the present mechanistic codon model has adjustable parameters for nucleotide mutation and for the strength of selective constraints, which can be tailored to specific genes.
Finally, with the following properties, each of the docking simulations were performed for 100 runs: Lamarckian Genetic algorithm[32] as the searching algorithm, 25,000,000 number of evaluations, population size of 250, 50,000 number of generations with the rates of 0.8 and 0.02 for mutation and for crossover respectively.
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For cases diagnosed above age of 60 years the ORs were 2.4 (P=0.0009) for NBS1 mutation, 1.8 (P<0.0001) for a CHEK2 mutation and 0.7 (P=0.6) for BRCA1 mutation.
The ORs for early-onset prostate cancer were 3.1 (P=0.003) for NBS1 mutation, 2.3 (P<0.0001) for a CHEK2 mutation and 1.9 (P=0.9) for a BRCA1 mutation.
Codon replacement were TCT172GCT for SA mutation and TCT172GAA for SE mutation.
Several F3 lines segregating for the cop9-1 mutandon and homozygous for PIN2-eGFP were selected.
These plants were assumed to be homozygous for the soh715 mutation and used for initial mapping of the mutation.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com