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Mutation and complementation implicate each gene in O-antigen expression but, as their putative sugar nucleotide substrates are not currently available, biochemical characterisation of WbmF, WbmG and WbmH is impractical at the present time.
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This could be attributable to the fact that they performed the mutations and complementation study in a wsc1 ∆ mid2 ∆ double-mutant that exhibited a severe lysis defect at all growth temperatures in absence of osmotic support.
It is demonstrated that the spliceosensor flies robustly respond to the presence of the DM1 mutation and to human MBNL1 complementation, whose loss of function explains most of the splicing alterations shown in this disease.
All plants tested were more susceptible than the resistant Kas-1 ecotype and complementation of the atl9 mutation restored the wild-type defense phenotype (data not shown).
The strategy involves combining a sequential mutagenesis scheme to reduce library size with structurally stabilizing mutations, chaperone complementation, and reduced temperature of gene expression.
Genetic mapping and complementation tests, utilizing the bleach sensitivity phenotype, assigned 27 independent mutations to nine complementation groups.
Df(1 C128 does not delete the FasII gene, and we confirmed that the line does not harbor a FasII mutation by complementation testing.
To confirm the effect of the ΔmelC mutation, a complementation test was performed by inserting the melC operon into the chromosome of YU5.
The mac33 mutation representing complementation group 1 (Table 2) caused an obviously blistered (Bli) phenotype.
To narrow down the genomic site affected by the mutation, intragenic complementation crosses with the aforementioned deletions were analyzed.
Initially we attempted to clone the mutation by complementation using the same protocols we used with Y857.
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