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Two cell lines generated from glioblastoma samples included in our mutational screen were also subjected to the mutation analysis we performed.
The mutation analysis we performed for our motif might have a different outcome when conducted within the folds of a different protein.
For the LAMC2 mutation analysis we sequenced overlapping cDNA fragments spanning the entire open reading frame in each two affected and non-affected sheep.
Furthermore, by mutation analysis we showed that a CRX binding element located within the 134 bp Nxnl1 fragment is necessary for promoter activity, thereby implicating CRX in the cell type-specific regulation of RdCVF expression.
In the full mutation analysis we detected two missense variants and eight intronic polymorphisms.
To simplify mutation analysis, we built a web interface for variant analysis using Delila software as the processing backbone.
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For EGFR mutations analysis, we used the EGFR Scorpions kit (DxS, Manchester, UK), which combines Scorpions amplification refractory mutation system (ARMS) and Scorpions technology, to detect mutations in Real-time Polymerase Chain Reaction (PCR) reactions.
On the basis of structural information and mutation analysis data, we identified candidate positions for accumulative histidine substitutions to cause electrostatic repulsion under acidic conditions.
To validate the mutation analysis (HRM), we used 49 DNA samples carrying known mutations and 48 wild-type controls (Table 2).
A possible confounding factor for intermolecular correlated mutation analysis is that we cannot be sure that the predicted orthologs in all the various species that we analyze do indeed interact.
Using a combination of high density SNP arrays and mutation analysis by sequencing, we have identified sequential genetic alterations, both known and novel.
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