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The methods for DNA isolation and mutation analysis have been described elsewhere.
Patients and mutation analysis have been described in Zhang et al. (2007).
The details of all the primers used for mutation analysis have been provided in Additional file 2: Table S7.
Most of the applications of IT for splicing mutation analysis have involved predominantly rare diseases, as well as some low frequency variants associated with more common genetic conditions.
In vitro systems, transgenic models, and mutation analysis have been used to great effect to characterize the bipolar cells' differing glutamate receptors, signaling proteins, and ion channels (Hanna & Calkins, 2007; Dhingra et al, 2008; Audo et al, 2009; Peachey et al, 2012; Ray et al, 2014).
In the future, frequency-based methods in cancer mutation analysis have the potential to draw from the vast experience accumulated in the field of evolutionary genetics (F ay and W u 2000; K reitman 2000; N ielsen 2005; E yre- W alker 2006).
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In many studies in lung cancer, but also in other cancers, EGFR mutation analysis has been restricted to the exons known to be frequently mutated.
To systematically evaluate how LATS1/2 genes play a critical role in human cancer, a mutation analysis has been carried out.
Single-point mutation analysis has often resulted in perplexing results, which are difficult to simulate using computation.
A rapid and accurate technique for assessing galactocerebrosidase activity and performing DNA mutation analysis had been developed.
Despite its high specificity, our meta-analysis shows that BRAFV600E mutation analysis has a low sensitivity in diagnosing papillary thyroid cancer in thyroid nodules.
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