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In addition, given that non- KRAS-mutated tumors include a distinct subset characterized by BRAF mutation, analyses were also performed to compare KRAS-mutated tumors with both BRAF-mutated tumors and the remaining subset of colorectal carcinomas, with observed neither somatic oncogene mutation.> -wrap-foot> Abbreviations: MMR mismatch repair, SD standard deviation.
Genomic DNAs for germ line mutation analyses were obtained from the buffy coat and resected tumor.
Mutation analyses were performed by PCR/sequencing.
Positive results from both mutation analyses were confirmed by sequencing.
Immunohistochemistry and mutation analyses were used to evaluate PTEN and PIK3CA, respectively.
p53 mutation analyses were conducted as described by Ko et al. [ 20].
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Genome-wide cancer mutation analyses are revealing an extensive landscape of functional mutations within the noncoding genome, with profound effects on the expression of long noncoding RNAs (lncRNAs).
Therefore, the success rate of DNA preparation for mutation analyses was >95%.
In clinical practice, mutation analyses are performed from a tissue sample available, preferably from a recently detected and resected metastasis.
A further obstacle for the interpretation of large-scale somatic mutation analyses is that fitness effects of the vast majority of mutations are unknown.
In clinical practice, mutation analyses are performed from a tissue sample available, preferably from a recently detected biopsied or resected metastasis.
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