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Like the DHFR N23PP mutant, this mutation ablated detectable exchange on the microsecond to millisecond time scale.
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Our mutational analysis of HD2 revealed multiple residues in this domain that are critical for β-catenin binding in vitro, including three leucine residues whose mutation ablated binding.
It is likely that this splicing mutation ablates Cbl's ubiquitin ligase activity, thereby over-stimulating Ras/MAPK signaling.
The Nnt mutation ablates a protein expressed in mitochondria of many tissues [ 28] and thus is likely to interact with a large number of other targeted mutations.
Each of the mutations ablates the ability of Vilya to interact with Mei-P22.
Although the S9A (serine 9 to alanine) mutant was phosphorylated identically to WT-Puma, the S10A mutation completely ablated the lower phosphorylated band without affecting the upper band.
In future work, it would be interesting to test how a knockin mutation that ablated dimerization motif dimerization pocket interaction affected channel function.
The employed analytical approach revealed the presence of low-frequency mutations not detectable by other procedures.
At a frequency of 0.3%, only one of the introduced mutations (A3278G) was detectable.
Interestingly, the effect of the R44A L45A mutation on virion release was additive with the S52/56N mutation, which ablates the binding of Vpu to β-TrCP, despite that both mutations impair the apparent degradation of BST2.
In contrast, yeast engineered with a CDC19 point mutation that ablates FBP-based regulation fail to accumulate phosphoenolpyruvate.
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CEO of Professional Science Editing for Scientists @ prosciediting.com