Sentence examples for mutating the sequence from inspiring English sources

By mutating the sequence of this ligand, a "secondary" library was generated, whose panning on HER2-positive cells isolated a phage-borne peptide with increased specific binding to HER2 (phage NL1.1).

DNA methylation changes are involved in epigenetic regulation mechanisms, which allow alteration of gene function without mutating the sequence.

To confirm that the reduction in red fluorescence observed following overexpression of miR-212 is mediated through the predicted target site, we tested whether this effect could be prevented by mutating the sequence of the site to disrupt binding to the miR-212 seed region.

Under less harsh conditions, in which some proteins can be tricked into being unfolded in the absence of denaturant (for example by mutating the sequence or changing the pH), the unfolded state has been shown to contain significant native-like interactions formed in concert with substantial numbers of both native and non-native side chain contacts [16,17].

The EOCT-mutpL construct was obtained by mutating the sequence of the A2 region containing the Octamer sites (underlined): C ATTAACATT CCCATTCAGT GCAGGTTTGA G ATGCTAATT TAGGAGCTTG AG ATGCTAAA into: C A GG A TTT TT CCCATTCAGT GCAGGTTTGA G A AAA T CC TT TAGGAGCTTG A GA AAA T CC A (mutated nucleotides are in bold).

A sequence of length 800bp was generated for the tips of the tree, starting with an ancestral sequence at the root and mutating the sequence along the branches using the Jukes-Cantor substitution model [ 16] with a mutation rate of 0.005.

In a mechanistically driven approach, RNA-IN and RNA-OUT variants were generated by mutating the sequences known to govern the specificity of the interaction.

RIP mutates the sequence of repeated DNA and over time renders the affected regions unrecognisable by similarity search tools such as BLAST.

A specific ancestral sequence is generated from the probability distribution, and the simulation mutates the sequence down the given tree topology according to the given substitution and mutation location models until a sequence has been generated for each leaf of the tree.

The forward primer was adjacent to the polymorphism and mutated the sequence to introduce an EcoRV restriction site in the A allele but not in the G allele such that in subsequent EcoRV digestion (37°C, 20 h), followed by agarose-gel electrophoresis, the A allele was restricted and the G allele remained uncut.

This construct was generated by mutating the nucleotide sequence that targets the shRNA of Ack1 but maintaining the amino-acid sequence of the protein.