Sentence examples for mutating the putative from inspiring English sources

Moreover, when mutating the putative active-site cysteine at position 313 to serine, covalent binding to the electrophile is abolished.

As shown in Figure 4E, mutating the putative binding site on the UTR increased luciferase activity significantly, again suggesting that the down regulation of CXCL12 is effected by miR-886-3p miR-886-3p miR-886-3pg throughputative bindirectite.

Because we were unable to alter TMEM16A-CaCC activity by inhibiting CaM function, could activate TMEM16A-CaCCs with barium, and could not shift channel EC50 by mutating the putative CaM-TMEM16A binding interface, we conclude that CaM is not responsible for the calcium sensitivity of TMEM16A-CaCCs.

To determine whether β-TRCP recognizes either ZNRF3 or RNF43 in a degron-dependent manner, we mutated the putative degron motifs (ESG and/or SSG in ZNRF3; DSG in RNF43).

Mutagenesis PCR was performed to mutate the putative ApiAP2 binding site at −825/−818 of the 103464 promoter from TAGAACAA to TTATTATT.

One of the important signalling pathways induced by TNFα results in the activation of transcription factor NF-κB. Therefore we mutated the putative NF-κB site in optineurin promoter and analysed the effect of this mutation on reporter activity.

Moreover, when we mutated the putative NES of LC3, EGFP-LC3 mNES was still found in both the cytoplasm and nucleus, a result inconsistent with a functional role of the NES.

Site-directed mutagenesis (Stratagene, Milan, Italy) or a two-round, PCR-based, site-specific mutagenesis approach were performed to mutate the putative Estrogen Receptor Response Elements (EREs) within the FLT1-T promoter construct.

Quickchange mutagenesis was performed on the entry clone containing the sup-26 3′ UTR using the primers CATCCACCGTTCCGTCATCGTCG and CTGCCGAGGAAAGGAGAATGAGTG to mutate the putative mir-35 family binding site.

To further confirm that the putative catalytic activity of Fam20A was not required for the increase in Fam20C-dependent substrate phosphorylation, we mutated the putative metal binding Asp of Fam20A (D430A).

To confirm this, we mutated the putative PD-binding site in each reporter construct to generate PRO1m-tk-luc, PRO2m-tk-luc, PRO3m-tk-luc, and PRO4m-tk-luc and then tested the responses of each to PAX3-FKHR expression.