Sentence examples for mutating the phosphorylation from inspiring English sources

We also hypothesise that mutating the phosphorylation sites of Ft (so Ft cannot be phosphorylated by Fj) will result in a reduction in binding affinity and therefore a reduced stable amount.

10.7554/eLife.05789.014 Figure 4. Effects of mutating the phosphorylation sites in Ds and Ft on in vivo stability.

This phosphorylation is important for AP2 function in vivo because mutating the phosphorylation site inhibits transferrin uptake (Motley et al., 2006; Semerdjieva et al., 2008).

For example, our first conclusion is tested by mutating the phosphorylation sites of Lsk1 to a phosphomimmick or non phosphorylatable residues, which consistently uncouples the regulation of the S2 kinase from the MAP kinase.

Based on previous experiments where phosphorylating Ds reduces the binding affinity between Ft and Ds (Brittle et al., 2010), we are able to hypothesise that mutating the phosphorylation sites in Ds, thus preventing Fj phosphorylation, should improve binding affinity and therefore increase the stable amount of bound protein.

However, the Act-ds- S>Dx3 -EGFP stable fraction is the same as Act-ds-EGFP (E ), intensity levels are similar (E′ ) and stable amounts are not significantly different (E″ ) suggesting that mutating the phosphorylation sites has not been successful or does not have any additional effect (see text).

In the present study we mutated the phosphorylation sites that we had shown were responsive to contraction (Ser, Ser and Ser), as well as Thr, an AMPK consensus site [ 21].

However, mutating the GluR1-S845 phositerylation site does not block LTP [32].

Mutating the Ds phosphorylation sites resulted in a significantly improved binding ability, however, this improved ability was lost when Ft was also not phosphorylated.

Therefore, as an approach to assess the role of PKA-dependent phosphorylation of RhoA in the activation of NHE1, we mutated the PKA phosphorylation site, serine188, to alanine to create a PKA phosphorylation dead (pd) RhoA mutant [11], [12].

To test whether the effect of Pkh-kinase activity on eisosome assembly and organization is directly due to phosphorylation of Pil1 rather than to an indirect effect, we next mutated the mapped phosphorylation sites to either alanine, which cannot be phosphorylated, or to negatively charged aspartate, which mimics phosphorylated serine or threonine.