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The team from Duke University in North Carolina created autistic mice by mutating the gene which controls production of the protein, Shank3.
An irrE library was constructed by randomly mutating the gene, and this library was then selected for tolerance to ethanol, butanol and acetate stresses.
At the genetic level, interactions between active nodes involve mutating the gene for an upstream SSTF and observing changes in the expression of a target SSTF.
These manipulations can be done pre-translationally by mutating the gene sequence (e.g. [ 5]) or fusing it with a stabilising partner (e.g. [ 6 ]).
Directed evolution normally involves mutating the gene that encodes the protein of interest, selecting the genes that produce the most promising proteins for another round of mutation, and repeating the process until the desired protein function is achieved.
Likewise, molecular perturbation of the circadian clock (i.e., altering the Clock, Bmal1, Per1, Per2, Cry1, or Cry2 expression via genetic manipulations including deleting or mutating the gene of interest to affect the levels of functional protein produced) disrupts normal circadian behavioral rhythms (Antoch et al. 1997; Bunger et al. 2000; van der Horst et al. 1999; Zheng et al. 2001).
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When mutated, the gene causes a tumor, but one that the rat's immune system can attack and destroy.
When mutated, the gene turns people into extreme early birds, rising, say, at the ungodly hour of 4 00 a.m.
Therefore, max min mutation operator selects subsystems with the highest reliability and the lowest reliability among the all subsystems and then mutates the gene values of each selected subsystem randomly.
His team mutated the gene encoding a protein that puts the finishing touches on Lipid A, a molecule in the bacterial cell wall that triggers inflammation.
But oddly, the secretion still took place even when they drastically mutated the gene--a resilience not found in any known secretion signal.
More suggestions(15)
mutating the sequence
mutating the CaM-recognition
mutating the core
mutating the tax
mutating the spacer
mutating the structure
mutating the text
mutating the polypeptide
mutating the leucine
mutating the filament
mutating the set
mutating the beginning
mutating the dEndoA-BAR
mutating the acceptor
mutating the arginine
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