Sentence examples for mutating the core from inspiring English sources

Mut-RE-III was obtained by mutating the core binding sequence AGGTTGCA.

Only small perturbations to the backbone, 1 2 Å, were needed to entirely mutate the core.

Based on the Transfac motif and literature research, we mutated the core nucleotides of the Pax2 and Sox2 binding sites by designing primers that contained the Pax2 binding core GTGAATAG mutated into TCAAACAT or the Sox2 binding core ACAAAA mutated into GTGCTC.

To characterize the specific interaction between the W-box and TaWRKY and TaRAV, we mutated the core W-box motif "TGAC" to "TG GC", "T AAC" and "T TTT".

Mutating the "TGA" core of the motif completely abolished the binding.

Amplicons were separately cloned into the pGEM-T vector and sequentially transferred into pGL3-TATA using KpnI, XhoI and SpeI restriction enzymes thereby mutating the −110 LEF1/Tcoreore sequence CTTTGA into ACTAGT.

To investigate the functional relevance of our findings, we mutated the DUX4 core motif present in FRG1 Peak1 region.

To investigate the importance of this sequence, we mutated the three core bases of the Ets site either singly or in combination within the context of the 508 bp reporter.

This structure appears to be determined by the protein factors that bind to core X, because mutating the Abf1 and ACS binding sites within core X disrupts the structure.

The contribution of the identified CRX binding elements to NXNL1 promoter activity was assessed by mutating the TAA in the TAAT core recognition site to CCG in the human and mouse −205/+57 and −353/+51 promoter constructs, respectively.

Mutating the conserved five amino acid core EFxxP or the highly conserved phenylalanine residue alone confirmed that these amino acids in both PAM2L motifs are crucial for the interaction with Rrm4.