Sentence examples for mutating the active from inspiring English sources

Mutating the active site cysteine (C277S) was already frequently employed to abolish TG2 activity (Akimov et al. 2000; Akimov and Belkin 2001b; Balklava et al. 2002).

The aminoacyl-tRNA synthetase (RS) is engineered to recognize unnatural amino acids by mutating the active site, based on a known structure [6], [7].

Mutating the active site does not impact orthogonality so the evolved pairs are highly unlikely to cross-react with the endogenous tRNA/RS pairs of their mammalian hosts.

We used cdc9-NΔ60 (which lacks the PIP [PCNA-interacting peptide] motif; see Figure 5A cartoon) and two ligase-null mutants, cdc9-K419A cdc9-K598A598A (mutheing the active center).

By mutating the active site nucleophile of ThnT from Thr to Cys, we prepared a chemically stable variant that was capable of undergoing a very slow N → S acyl shift.

In contrast, mutating the active site Cys (corresponding to Cys at the active site in human Capn3) to a serine Capn3b-C120SS) abolished the ability of the mutant Capn3 to downregulate the p53 level induced by the injection of capn3b-MO.

Binding of phenylalanine in the active site is effectively abolished by mutating the active-site residue Arg270 to lysine.

Moreover, when mutating the putative active-site cysteine at position 313 to serine, covalent binding to the electrophile is abolished.

To ablate catalytic activity but potentially retain the correct fold, we mutated the two active site Asp residues to Ala.

We now have generated a structural model for human QC based on the aminopeptidase fold (pdb code 1AMP) and mutated the apparent active site residues to assess their role in QC catalysis.

EndoS2 was mutated in the active site through site-directed mutagenesis where the catalytically active glutamate residue was mutated to leucine, creating the enzyme EndoS2(EndoS2.