Sentence examples for mutating the PD-binding from inspiring English sources

As shown in Figure 4B, mutating the PD-binding site in PRO2 abolished its transactivation by PAX3-FKHR.

Mutating the PD-binding site in PRO1 and PRO3 not only abolished the reporters' transactivation by PAX3-FKHR but also decreased their basal activities (65% decrease for PRO1 and 69% decrease for PRO3), possibly because the basal activation of PRO1 and PRO3 is primarily mediated by mechanism independent of PAX3-FKHR.

To confirm this, we mutated the putative PD-binding site in each reporter construct to generate PRO1m-tk-luc, PRO2m-tk-luc, PRO3m-tk-luc, and PRO4m-tk-luc and then tested the responses of each to PAX3-FKHR expression.

Mutating the miR-500a-3p miR-500a-3p miR-500a-3p 3′UTR reversed effect of miR-500a-3p (Figs. 6G and S5).

Mut-RE-III was obtained by mutating the core binding sequence AGGTTGCA.

This expression could be eliminated by mutating the SOX9-binding site of CRE2.

Mutating the miR-211 binding site seed sequences at the KCNMA1 3'-UTR abolished target cleavage.

While mutating the RGD and heparin binding sites had little effect, altering the IGF binding site abolished its biological activity.

To examine whether the interaction between miRNAs and their target sequences direct or indirect, we mutated the miRNA binding sites with one or two nucleotide mutations (Table 2).

To test this conjecture, we mutated the Pho4 binding site at UASp2.

To do so, we used site directed mutagenesis to mutate the FOXO1a binding site in the promoter of TXNIP.