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Mutating the CaM-recognition element (R3) reduces colocalization significantly (blue).
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Because we were unable to alter TMEM16A-CaCC activity by inhibiting CaM function, could activate TMEM16A-CaCCs with barium, and could not shift channel EC50 by mutating the putative CaM-TMEM16A binding interface, we conclude that CaM is not responsible for the calcium sensitivity of TMEM16A-CaCCs.
The p65/RelA D361E mutant was generated by mutating the INFD putative consensus recognition site for caspase-6.
To mutate the CpaAII recognition site of pSY6catP using three silent point mutations, a gBlock was synthesized in which codons 521 (AGU → UCU; Ser) and 523 (GCU → GCC; Ala) were mutated within the ltrA coding sequence.
We added different modules from other systems, deleted most redundant sequences, and mutated the NotI recognition site between the connection region of pVS1 REP and STA from pCAMBIA1300.
To overcome the uncharacterized restriction barrier, we attempted to mutate the unknown restriction recognition sequence in the 334 bp inhibitory region of pSY6catP.
However, mutating the leucine to tyrosine did not affect recognition specificity.
That furthered my sense of mutating the genres.
Subject DR with Pro1199Leu substitution has mutated the last nucleotide in the recognition sequence of BsaHI (AcyI): GACGCT* vs. GRCGYC.
We know steroids mutated the records.
The N-terminal CaMBD of AC8 appears to act as a CaM tethering site, because exogenous CaM is needed to permit stimulation by Ca2+ when the N-terminus is truncated, and even when the critical C-terminal CaMBD is mutated, the consequences are not apparent unless the N-terminus has been either deleted or mutated so that it can no longer bind CaM.
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