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Each generation, a network had a 1% probability of mutating one of its weights.
We identified three potential YxxΦ motifs within TLR7, and mutating one of these (Tyr-892) disrupted the interaction with AP-4.
A 100 mV change in reduction potential was observed upon mutating one of the Cys residues in thioredoxin-like ferredoxins that have five Cys residues.
Thus, although we did not mutate N26 directly, we have nevertheless changed its local mobility by mutating one of its hydrogen bond partners, I28.
Mutating one of the Cys residues to Ser still gives LMCT bands in ferric form, but with the peaks shifted to higher energy together with some changes of intensity, consistent with a decreased S to Fe(III) LMCT contribution.
Mutating one of the two bridging cysteines to serine, Cys216Ser, in C cO from Pa .denitrificans resulted in a type 1 blue copper site with four-line EPR hyperfine splitting rather than the seven-line EPR signal observed in the CuA site, and only retained below 1% of the wild-type activity.
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These three hits are consistent with a possible Mg-dependent ribonuclease function, and this prediction was subsequently demonstrated in vitro and in vivo; moreover, when we mutated one of the predicted Mg-binding aspartic acids to asperagine, the nuclease activity was abolished, confirming the predicted active site location (Matsushita et al., 2009).
By mutating one residue of the β2 subunit to that of the α4 subunit, we find that cytisine can activate the A2B3 receptor.
The specificity of binding was further illustrated by mutating one base on either side of the A or G within the respective oligos.
Regulatory roles of each uAUG/uORF may be studied by mutating one or more of the uAUGs to mitigate repression.
Mutating one or both of the Zn2+-binding His residues in ADR1a-SecM (L = 24) to Ala returns fFL to baseline.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com