Sentence examples for mutated using a from inspiring English sources

For the mutant, the myocardin-binding sites were mutated using a mutagenesis kit (Stratagene).

The others in M i are mutated using a random model based on the observed characteristics of the glioblastoma data.

The best offspring resulted from this process is mutated using a pull move transformation within a random hill climbing (RHC) procedure.

A second sbf1 site further upstream was initially mutated using a site-directed mutagenesis kit (Agilent Technologies, Wilmington, DE), prior to cloning.

Human CLR with an N-terminal haemagglutinin (HA) epitope tag, human RAMP2 with an N-terminal FLAG epitope tag (Qi et al., 2013) and untagged human RAMP3 were mutated using a method based on the Quik Change II site-directed mutagenesis kit (Stratagene, Cambridge, UK) as described previously (Conner et al., 2005; Bailey and Hay, 2007).

For that purpose, miRNAs were transfected into the cell lines MCF-7 (luminal-like, ER-positive) and BT-474 (ERBB2-amplified, ER-positive, TP53 mutated) using a library of pre-miR constructs.

These proteins had been mutated, using an alanine-scanning approach, at 10 surface-exposed lysine residues of the C-terminal domain.

Each individual, VMs-to-PMs assignment, resulting from the selection is mutated using the mutation probability.

To label the proteins with fluorescent probes, MscL was mutated using site-directed mutagenesis such that a residue at the desired position was replaced by a cysteine.

Cygb pET28a plasmid was mutated using site-directed mutagenesis based on a modified Agilent Quikchange II method to give the H81V and H81Y mutation.

A strain of Streptomyces was mutated using N-nitroso-N-methyl urethane, and this new strain produced a different, red-colored antibiotic.