Sentence examples for mutated to serine from inspiring English sources
Second, the five cysteine residues of telethonin were mutated to serine to avoid severe problems with cysteine oxidation.
In order to study effects of the mismatched disulfide bond, Cys-VL85 and Cys-VL84 of FV57 were mutated to serine to construct mutants FV57VL85S and FV57VL84S.
We examined the frequently used Nostoc punctiforme Npu DnaE intein after the C-extein cysteine nucleophile (Cys+1) was mutated to serine or threonine.
Furthermore, the cysteine at VL85 of ds-FV57 was mutated to serine to construct ds-FV57VL85Ser in order to avoid potential mis-formed disulfide bonds which would alter the affinity of the scFv.
Third cysteine of MtbDGC at position 406 was mutated to serine amino acid using PCR based approaches.
The Cys406 was found to be free and interestingly when mutated to serine the protein was found to be inactive.
The two cysteines of PRL-3 CCVM were mutated to serines (Fig. 1B) to specify the exact crucial site in tumor migration.
These cysteines were mutated to serines and additional cysteines were introduced at a distance of four (Tlg1M5) or ten (Tlg1M6) amino acids (Figure 7A) from their original position.
The highly conserved 9′ leucine residues in α4, β3 and δ subunits were mutated to serines, producing α4(L297S), β3(L284S) and δ(L288S).
To determine the importance of the individual cysteine residues identified via mass spectrometry in photoaggregation, we constructed HγD-Crys mutant constructs with cysteines of interest mutated to serines.
The plant thioesterases were still highly active when their cysteines were mutated to serines, while the thioesterases from rat and chicken livers retained up to 90% of the activities when their serines were substituted with cysteines [ 26- 28].
