Sentence examples for mutated to prevent from inspiring English sources

I selected a CRISPR/Cas9 target site that would produce a DSB 17 bp 3′ from the e2123 point mutation and designed an 80mer repair oligo with the PAM silently mutated to prevent recleavage of the site in an edited animal.

PAM sequences of the targets in the arms were mutated to prevent re-cutting after successful integration.

To explore how RGKs influence gating kinetics, we separated the roles mediated by the Ca(2+) channel accessory beta subunit's interaction with its high affinity binding site in the pore-forming alpha 1C) subunit (AID) from its other putative contact sites by utilizing an alpha 1C *beta3 concatemer in which the AID was mutated to prevent beta subunit interaction.

In the current study, we have used FasL-deficient mice as well as mice from a gene-targeted line in which the FasL metalloproteinase cleavage sites were mutated to prevent cleavage of the membrane-bound protein.

Previous work identified one strategy where a gene called UBP6 is mutated to prevent it from working correctly.

The D110A,K220RGRK2 construct, which is mutated to prevent both kinase activity and Gαq/11-binding, markedly attenuated ETAR desensitization.

To corroborate the effect of multisite phosphorylation on DGCR8 protein levels, we expressed in HEK 293T cells constructs containing subsets of residues mutated to either prevent (Mut23 and Mut14 have 23 and 14 sites mutated, respectively) or mimic (Mim11 and Mim23 have 11 and 23 sites mutated, respectively; Table S2) phosphorylation.

In an attempt to better define the physiological roles that C-terminal post-translational modification play we generated knockin mice in which Ser was mutated to alanine to prevent phosphorylation or Cys was mutated to serine to prevent farnesylation.

Consistent with our working hypothesis, LTD was blocked in cells co-transfected with p47phox shRNA and an shRNA-resistant mutant form of p47phox with all four residues mutated to alanine, to prevent their phosphorylation (after LFS: 95.1 ± 6.8%, p = 0.725 vs. control input, Fig. 4a).

The endogenous Cys190 was mutated to Ser to prevent labeling at this position.

In one mutant, the Uba2 catalytic Cys177 was mutated to Ala to prevent formation of a Uba2∼Sumo intermediate, but the ufd was wild-type.