Sentence examples for mutated the two from inspiring English sources

These results suggest that AP-2α also regulates PP2A-Aα To further confirm the AP-2α control on PP2A-Aα gene promoter, we again mutated the two AP-2α binding sites either individually or in combination (the mutation of the two AP-2α sites are shown in Fig. 5A) and assayed the relative luciferase activity from the two constructs in both ARPE-19 and FHL124 cells.

In this study, we mutated the two sites, expressed four resulting mutants in Escherichia coli and characterized.

wo mutated the two cysteinos to serines, individually and concurrently.

Therefore, we mutated the two UIMs in the Atx3(1 263) fragment in order to compromise their functionality [Atx3(1 263)(S236A,S256A)].

To test the effects of TR on CTCF mediated enhancer blocking, we mutated the two half sites of the F2 element, which is characterized by an everted palindrome spaced by 6 nucleotides.

Next, we mutated the two miR‐9 sites to generate the CBX7‐3′UTR 9.1/2 reporter.

We mutated the four aforementioned residues to alanine and generated four stable mutant ArgRSs (D118A, Y313A, D317A and R324A).

To identify the CK2 phosphorylation sites, we mutated the four CK2 consensus sites to Ala either individually or in groups (Fig. 2D I).

To better understand the relationship between Elm1 and SBF activity, and the role of phosphorylation of Elm1 by Cdc28, we performed subsequent analysis of MatA, Δelm1, and elmT551A, a strain in which we endogenously mutated the threonine in the single Cdc28 consensus site found in Elm1 to prevent its phosphorylation.

We then mutated the three critical tyrosines (Tyr232, 253 or 257) individually to glutamic acid, which mimics phosphorylation, in the BCR63-ABLKD context and found that each Y/E substitution alone is sufficient to block the nuclear import of this kinase-defective BCR63-ABLKD protein (Figure 5B).

We simultaneously mutated the six nonconserved residues to the corresponding UbcH5b residues.