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Finally, to further prove that ERK5 is a miR-143 target gene transient transfection experiments were performed using the 3'UTR region of ERK5 containing the putative miR-143 matching site, mutated or not, downstream of the luciferase open reading frame.
Sequences were considered mutated or not using the cutoff of 2% mismatch.
BrdU incorporation was analysed by immunofluorescence microscopy to determine the number of dividing cells after a 20-day treatment with HIV-proteins mutated or not (C).
Second, the combination of sparseness and binary data (mutated or not mutated) may generate difficulties in the Cox model fitting.
Whether Tehran's rats are mutated or not, some scientists have reported that certain rat populations are becoming resistant to poison.
The activity of each alternative promoter A, B and C, mutated or not in the NF-Kappa B binding sites, was increased in Nter-142Q compared to Nter-15Q neuronal-like cells.
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The regulatory effects of BRCA on GR were assessed in 146 serous ovarian cancer patients (28 pairs of BRCA1-mutated or not, 23 pairs of BRCA2-mutated or not, and 22 pairs with hypermethylated BRCA1 promoter or not).
Fresh tumor samples, adjacent normal ovarian tissues, ascites, and blood samples were obtained at the time of primary surgery before any chemotherapy or radiotherapy (28 pairs of BRCA1-mutated or not, 23 pairs of BRCA2-mutated or not, and 22 pairs with hypermethylated BRCA1 promoter or not).
Genes that are mutated at high frequency in diverse human epithelial tumours, including PTEN, EGFR, ERBB2, BRAF, RAS family genes, RB1 and APC, are either not mutated or are mutated at relatively low frequencies (<10%) in ccRCC.
The implication of this is that virtually all described XIAP mutations in XLP2 patients, irrespective of whether the mutated protein is expressed or not, will predictably cause impaired RIPK2 ubiquitylation and activation of NF-κB-mediated transcription in response to NOD2 stimulation (and most likely NOD1 stimulation; Fig 7G).
Ectopic overexpression of Cx43 (WT or mutated) did not influence cell size or proliferation (supplementary material Fig. S1B,C).
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