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In contrast to the stability of the epitope tags, the SV40 NLS sequence was rapidly mutated by a frameshift mutation that introduced negatively charged amino acids into the basic NLS.
To further ensure that NF-κB indeed mediates ET-1-induced COX-2 promoter activity through binding to its regulatory element within the COX-2 promoter region, the wild-type (WT) and mutated by a single-point mutation of the NF-κB binding site (mt-κB) COX-2 promoters were constructed (as illustrated in Figure 6E, upper panel).
In many fungi, duplicated DNA is actively mutated by a process called repeat-induced point mutation (RIP) [ 76].
The nonadjacent rbcL and rbcS genes in the selection plasmid were mutated by a standard error-prone PCR protocol with 0.015 and 0.1 mmol/L Mn2+, respectively (Joo et al., 1999).
In the episode, Spider-Man must face "The Lizard," who was a lab professor he was interning for until he was mutated by a serum designed to regrow his missing arm.
The irrE gene was mutated by a standard error-prone PCR protocol (see Materials and Methods).
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The segment gene is mutated by an exchange mutation operator, involving the exchange of two randomly selected genes.
The node gene is mutated by an integer mutation operator, which itself is replaced by another gene with a certain mutation probability.
He invented a race of creatures mutated by an apocalyptic nuclear war who, in order to survive, live inside robotic shells and are so convinced of their own purity that their object is to exterminate every other race.
The Daleks were the brainchild of the writer Terry Nation, as Jill Lepore writes in this week's magazine: He invented a race of creatures mutated by an apocalyptic nuclear war who, in order to survive, live inside robotic shells and are so convinced of their own purity that their object is to exterminate every other race.
Instead of Flint Marko's character, Cameron's "Sandman" (simply named Boyd) is mutated by an accident involving Philadelphia Experiment-style bilocation and atom-mixing, in lieu of getting caught in a nuclear blast on a beach.
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