Sentence examples for mutated at both from inspiring English sources

The pGL4.20 luciferase reporter plasmids containing either a wildtype Cebpb promoter fragment (−516 to −1) or a mutated version which is mutated at both FoxO binding elements (FBE1 (−234 to −225) and FBE2 (−208 to −199)) were generous gifts from Dr. Akiyoshi Fukamizu, and have been described previously [ 25].

In 70 90% of colorectal cancers with MSI, TGFBR2 is frameshift mutated at both alleles [17].

In AGS cells, CST1 cDNA was mutated at both R91R and N82S (AAT to AGT).

The BGLF4 SIM domains were mapped and when mutated at both the N- and C-terminal SIMs, the intracellular localization of the kinase shifted from nuclear to cytoplasmic.

Of the mutated K-ras genes in these patients, 92% were mutated at codon 12, 8% at codon 13, and none of them was mutated at both codons 12 and 13.

On the other hand, expression of the reporter construct mutated at both miR-26a binding sites of E2F7 3′-UTR (Mut1–2) was not sensitive to miR-26a translational repression.

These wild-type constructs were then mutated at one or both of the SBSs, using a mutagenic PCR primer to introduce a leucine-to-proline mutation.

Lehman and Harris observed that mouse PrP mutated at T182 alone or at both T182 and T198 in CHO cells failed to reach the cell surface but those mutated at T198 did.

Hence, in this work, we analyzed the functional repercussions of Tau-aggregation in optimal conditions, using Tau mutated at Proline 301 acting both as inducer and recipient.

Similarly, the HEC59 cells are mutated at different loci on both alleles of MSH2 and are deficient in repair activity (Umar et al, 1997).

To further dissect YB-1 binding within the 2a region we designed biotin-labelled oligonucleotides in which the YB-1-responsive elements (YREs) were mutated at -968, -940 or both sites.