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Exact(5)
As a negative control, a fragment in which the putative miRNA binding site was mutated at all 7 residues was inserted into the same pMIR-Report plasmid.
Although we tested NRPD1 or NRPE1 subunits mutated at all three aspartates of their Metal A sites, genetic evidence suggests that mutation of even one of these aspartates is sufficient to disrupt Pol V function.
hLECs were co-transfected with wild-type Prdx6 promoter linked to CAT or promoter constructs mutated at all Sp1 sites (Mut-1+2+3) along with pCMV-Sp1 expression construct (4 μg).
There was no competition when the Y-box reverse CCAAT motif was mutated at all five positions (Fig. 3a, lanes 7 and 8), nor with the unrelated HIV-1 κB sequence (lane 10).
TRIM5αrh8KR (K45R, K50R, K85R, K218R K284R, K371R, K372R, K372R) is mutated at all of the Lys residues found to be ubiquitinated by MS analysis as well as at lysine residues 44 and 371, selected for mutation based on close proximity to lysine residues 45 and 372.
Similar(54)
Of the molecular markers investigated (all genes mutated at a population frequency >1.7 % and frequent chromosomal imbalances) in the entire glioma dataset, 57 were significantly associated with overall survival.
iii) Mutation: The new created individual will be mutated at a definite point in the chromosome. .
Here, site-directed mutagenesis created Elk-1 mRNA mutated at a single site.
The promoter was mutated at a single TCF4/Lef1 binding site or both binding sites at a time.
In order to map the RNF10-binding motif within the SSE sequence, we analyzed a series of oligonucleotides in which 2 bases were mutated at a time.
TSC1, as well as TSC2, that constitute the mTOR regulatory tuberous sclerosis complex were found to be mutated at a combined frequency of 15%.
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