The establishment of an International repository containing CRISPR directed against all rice genes to generate knock-out mutants would be a helpful resource for speeding up gene discovery and validation.
Thus, M. tuberculosis phoP mutants would be better pre-adapted to persist in the host.
Being predominantly in the GTP bound state the Rab7 Cmutantstants would be expected to promote premature degradation of the TrkA containing signaling endosomes.
Using actin stress fibers as an indirect biological readout, we asked whether the RhoGAP activity of DLC1ΔPTB mutants would be affected.
Dpolv nonsense mutants would be found in so called constitutive exons expressed in most, if not all, tissues at all developmental stages.
With regards to the yeast deletion mutants, we predicted that the ssn6 and tup1 mutants would be similar, as acting as part of the same protein complex.
We therefore reasoned that any inhibition of endogenous wild-type IDH1 or IDH2 by transgenic IDH1 or IDH2 mutants would be apparent at this substrate concentration.
However, the morphological defects displayed in sexual and asexual reproduction made us question whether or not the ΔBAR1 mutants would be defective in differentiating infection-related hyphae [21].
Because of these circumstances, we reasoned that the degree of cleavage of the various PrPC mutants would be more reliably assessed by measuring C1 rather than N1.
However, many of the selected mutants would be unable to grow on defined medium due to fundamental problems in biosynthetic pathways.
Since it has been reported that phoP mutants are more susceptible to oxidative killing in vitro [22], we asked whether phoP mutants would be more virulent in CGD mice, and they were not.
